The MolTag Doctoral Program invites you to the following talk:
Title: Using Circular Dichroism (CD) Spectroscopy to Explore Protein Structure and Function
By: Robert W. Janes PhD, Senior Lecturer, School of Biological & Chemical Sciences, Queen Mary University of London
on: Friday, December 2nd, 2022, 10:30 a.m.
Location: UZA2, Josef Holaubek-Platz 2, 1090 Vienna; SE Room 2D 358
Host: Assoc.Prof.Dr. Anna Weinzinger
Abstract: Circular Dichroism (CD) spectroscopy is a valuable method for providing structural and functional information about both soluble and membrane proteins. This talk will describe a range of experimental methods and new software available for exploring, for example, the effects of mutations in proteins, of the binding of small molecules (ligands or drugs) or of macromolecules to proteins, and of establishing the stability of proteins. It will show methods, including software tools, available to aid in the interpretation of the spectral data for deriving new information on the structure and function of proteins.
Biography: Robert W. Janes obtained his B.Sc. in Chemistry at Royal Holloway College, University of London and his PhD in Crystallography from Birkbeck College, University of London, where he also did postdoctoral work. He is currently a Senior Lecturer in Biological Sciences at Queen Mary University of London, where he has held grants for many years from the UK Biological and Biotechnology Research Council in the areas of circular dichroism and bioinformatics. He is a Fellow of the Royal Society of Chemistry and the International Union of Pure and Applied Chemistry, and was a winner of the Jobin-Yvon Prize in spectroscopy.
Selected references for the talk:
Miles, Janes, and Wallace (2021) Tools and methods for circular dichroism spectroscopy of proteins: a tutorial review. Chem. Soc. Rev. 50: 8400-8413.
Ramalli, Miles, Janes, and Wallace (2022) The PCDDB (Protein Circular Dichroism Data Bank): A bioinformatics resource for protein characterisations and methods development. J. Mol. Biol. 434:167441.
Drew and Janes (2020) PDBMD2CD: providing predicted protein circular dichroism spectra from multiple molecular dynamics-generated protein structures, Nucleic Acids Research, 48, W17–W24.
Mavridis, and Janes (2017) PDB2CD: a web-based application for the generation of circular dichroism spectra from protein atomic coordinates. Bioinformatics 33: 56-63.
BOOK: Wallace and Janes (Editors) (2009) Modern Techniques in Circular Dichroism and Synchrotron Radiation Circular Dichroism Spectroscopy. IOS Press, Amsterdam.
Contact: office.moltag@univie.ac.at
